One of the first steps in selecting the appropriate statistical analysis method is determining if the variables to be compared are numerical or categorical. For example, if a study were to be done on blood pressure and patients were defined as having either high or low blood pressures, they are being evaluated according to categorical variables. If instead patients were defined by specific blood pressure ranges (systolic BP 100-109, 110-119, 120-129 etc), they would be evaluated according to numerical variables.
A t-test is ideal for comparing mean values (or averages) between two groups to determine if there is a significant difference between the two groups. One example of this would be to compare the numerical mean systolic blood pressure in men versus women. Importantly, the t-test can only be applied when the test statistic follows a normal bell-curve distribution.
ANOVA, which is short for analysis of variance, is a stastical model used to analyze the numerical differences among greater than three groups in a study. One application of this would be comparing the mean blood pressure between members of three or more ethnic groups.
When selecting a statistical test to compare numerical variables, it is important to remember that these tests are only useful when comparing mean values between groups, in which the numerical outcomes result in a normal bell-curve distribution.
The chi-squared test is used to determine if there is a statistically significant difference between frequencies or proportions of outcomes in one or more categories. For example, the chi-squared test would be appropriate to use to compare what percentage of multiple ethnic groups have high blood pressure. It is important to note that although percentage and proportion are numerical values, the variable actually being studied, in this example blood pressure, is defined as high or not high and is therefore categorical.
Fisher's exact test is a statistical test used to analyze differences in percentages of categorical outcomes between groups and is specifically used for small sample sizes, as opposed to the chi-squared test which is used to analyze differences in categorical outcomes between large groups. One example of this would be comparing the percentage of individuals with hypertension in a group of 20 women versus a group of 20 men.
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